Binding ofClostridium botulinumtype B toxin to rat brain synaptosome
نویسندگان
چکیده
منابع مشابه
High-affinity binding of Clostridium perfringens epsilon-toxin to rat brain.
125I-epsilon-toxin showed high affinity to rat brain homogenates and synaptosomal membrane fractions, having single binding phases with dissociation constants (Kds) of 2.5 and 3.3 nM, respectively. Treatment of synaptosomal membrane fractions with pronase and neuraminidase lowered the binding of the labeled toxin, whereas treatment with trypsin and phospholipase C did not. Heating of the fracti...
متن کاملSecretin: specific binding to rat brain membranes.
The binding of [125I]secretin to rat brain membranes was investigated. Radiolabeled secretin bound with high affinity (KD = 0.2 nM) to a single class of noninteracting sites. Binding was specific, saturable, and reversible. Regional distribution studies indicated that the specific binding was greatest in the cerebellum, intermediate in the cortex, thalamus, striatum, hippocampus, and hypothalam...
متن کاملBomebesin: specific binding to rat brain membranes.
The binding of a radiolabeled bomebesin analogue to rat brain membranes was studied. [125I-Tyr4]Bombesin bound with high affinity (KD = 3 nM) to a single class of non-interacting sites. Binding was specific, saturable (3.8 pmol of sites/g of wet tissue), and reversible. Regional and subcellular distribution studies showed that the density of sites was 7-fold greater in the hippocampus than the ...
متن کاملHigh affinity binding of tetanus toxin to mammalian brain membranes.
A sensitive binding assay was developed to study tetanus toxin binding to brain membranes in vitro. Binding of '2"I-tetanus toxin was linear with tissue content only at very dilute tissue concentrations, 0-20 ng membrane protein/200 pl for bovine cortex membranes. At these low tissue concentrations '261-tetanus toxin displayed high affinity, single phase binding with a dissociation constant (KD...
متن کاملCharacterization of cysteine sulphinate transport by intact rat brain cerebrocortical synaptosome fractions.
4. Binder, L. I . , Frankfurter, A. & Rebhun. L. I. ( 1985) J . Cell Biol. 101.1371-1378 5 . Wischik. C. M., Novak, M., Thogersen, H. C., Edwards, P. C., Runswick, M. J., Jakes, R., Walker, J. E., Milstein, C., Roth, M. & Klug, A. ( 1 988) /'roc. Natl. Acad. Sci. U.S.A. 85,4506-45 I 0 6. Bre. M. H. & Karsenti, E. (1990) C'eN Motil. C'ytoskel. in the press 7. Mitchison, T. & Kirschner, M. (1984)...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1990
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1990.tb03896.x